Research Paper Volume 15, Issue 9 pp 3356—3380
Highly multiplexed immune profiling throughout adulthood reveals kinetics of lymphocyte infiltration in the aging mouse prostate
- 1 Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA
- 2 Molecular Biology Interdepartmental Program, University of California, Los Angeles, CA 90095, USA
- 3 Division of Hematology-Oncology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
- 4 Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
- 5 Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, USA
- 6 Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA
- 7 Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
- 8 Current Address: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA
- 9 Current Address: Keck School of Medicine, University of Southern California, Los Angeles, CA 90095, USA
- 10 Current Address: Division of Cardiac Surgery, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Received: February 6, 2023 Accepted: April 17, 2023 Published: May 13, 2023
https://doi.org/10.18632/aging.204708How to Cite
Copyright: © 2023 Fox et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Aging is a significant risk factor for disease in several tissues, including the prostate. Defining the kinetics of age-related changes in these tissues is critical for identifying regulators of aging and evaluating interventions to slow the aging process and reduce disease risk. An altered immune microenvironment is characteristic of prostatic aging in mice, but whether features of aging in the prostate emerge predominantly in old age or earlier in adulthood has not previously been established. Using highly multiplexed immune profiling and time-course analysis, we tracked the abundance of 29 immune cell clusters in the aging mouse prostate. Early in adulthood, myeloid cells comprise the vast majority of immune cells in the 3-month-old mouse prostate. Between 6 and 12 months of age, there is a profound shift towards a T and B lymphocyte-dominant mouse prostate immune microenvironment. Comparing the prostate to other urogenital tissues, we found similar features of age-related inflammation in the mouse bladder but not the kidney. In summary, our study offers new insight into the kinetics of prostatic inflammaging and the window when interventions to slow down age-related changes may be most effective.