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Aging-US: MiR-513b-5p represses autophagy by targeting PIK3R3

07-25-2021

Aging-US published "MiR-513b-5p represses autophagy during the malignant progression of hepatocellular carcinoma by targeting PIK3R3" which reported MiR-513b-5p repressed liver cancer cell proliferation, migration/invasion, and induced apoptosis in vitro.

Crucially, miR-513b-5p attenuated tumor growth of liver cancer cells in vivo.

In the mechanical investigation, the authors identified that PIK3R3 mRNA 3′UTR was targeted by miR-513b-5p and miR-513b-5p suppressed PIK3R3 expression.

PIK3R3 overexpression partly reversed miR-513b-5p-mediated autophagy, proliferation, and apoptosis of liver cancer cells.

Consequently, they concluded in their Aging-US Research Output that miR-513b-5p repressed autophagy during the malignant progression of HCC by targeting PIK3R3. MiR-513b-5p may be applied as a therapeutic target for HCC.

Consequently, they concluded in their Aging-US Research Output that miR-513b-5p repressed autophagy during the malignant progression of HCC by targeting PIK3R3. MiR-513b-5p may be applied as a therapeutic target for HCC.

Dr. Rongjun Nie from The Guangxi Medical University said, "Liver cancer is a prevalent malignancy and the principal reason for tumor mortality globally, in which hepatocellular carcinoma (HCC) depicts 70–85% of the entire liver carcinoma weight."

As the previous studies, autophagy is a crucial process during liver cancer development and a potential therapeutic target for liver cancer therapy, but the mechanisms are poorly understood.

PIK3R3 acts as an oncogene of various cancers, containing glioma, lung cancer, and gastric cancer.

As several miRNAs are involved in the modulation of autophagy in HCC and based on the crucial role of miR-513b-5p in cancer development, the authors selected miR-513b-5p as an example to evaluate its function in autophagy during liver cancer progression.

Figure 7. Autophagy inhibitor 3-MA reverses miR-513b-5p-mediated liver cancer progression in vitro. (A–F) The HepG2 and Huh-7 cells were treated with miR-513b-5p mimic and 3-MA (5mM). (A and B) The analysis of cell proliferation using MTT assays. (C and D) The analysis of cell apoptosis using flow cytometry. (E and F) The analysis of cell migration/invasion using transwell assays.

In the present study, they were interested in the miR-513b-5p function in the modulation of autophagy during liver cancer progression.

They demonstrated that miR-513b-5p attenuated autophagy during the malignant progression of liver cancer by targeting PIK3R3.

The Nie Research Team concluded in their study, "miR-513b-5p repressed autophagy during the malignant progression of HCC by targeting PIK3R3. MiR-513b-5p may be applied as a therapeutic target for HCC."

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DOI - https://doi.org/10.18632/aging.203135

Full Text - https://www.aging-us.com/article/203135/text

Correspondence to: Rongjun Nie email: nrj2001@163.com

Keywords: hepatocellular carcinoma, autophagy, progression, miR-513b-5p, PIK3R3

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Cancer and aging are two sides of age-related tumorigenesis.

The mission of the journal is to understand the mechanisms surrounding aging and age-related diseases, including cancer as the main cause of death in the modern aged population.

The journal aims to promote 1) treatment of age-related diseases by slowing down aging, 2) validation of anti-aging drugs by treating age-related diseases, and 3) prevention of cancer by inhibiting aging. (Cancer and COVID-19 are age-related diseases.)

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