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Research Paper|Volume 17, Issue 1|pp 203—216

Comprehensive analysis of LMNB2 in pan-cancer and identification of its biological role in sarcoma

Yonghui Guo1,2, Min Zhang3, Yingrui Luo4, Yingshi Li5, Yanxia Xu6, Nisha Wang7
  • 1Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China
  • 2Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, Guangdong Province, China
  • 3GuangDong Second Province General Hospital, Neurosurgery Department (MH), Guangzhou, Guangdong Province, China
  • 4Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province, China
  • 5Southern Medical University, Guangzhou, Guangdong Province, China
  • 6Department of Radiation Oncology, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, Guangdong Province, China
  • 7Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province, China
* Equal contribution
Received: September 18, 2023Accepted: December 27, 2023Published: July 8, 2024
https://doi.org/10.18632/aging.205962

Copyright: © 2024 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Backgrounds: Sarcoma (SARC) is a mesenchymal tumor which often responds poorly to systemic therapy. It is therefore important to look for possible biological markers that could tell the prognosis and the progression of SARC.

Methods: A combined evaluation of the Cancer Genome Atlas (TCGA) and genotypic tissue expression (GTEx) portal was used to analyzeLMNB2 expression level in different types of cancer. Kaplan-Meier survival analysis was performed to examine LMNB2 predictive value in over-all survival rate and disease-free survival rate. The association among LMNB2 expression level and immune cell infiltration, microsatellite instability (MSI) and tumor mutational burden (TMB) were analyzed. GO and KEGG enrichment analysis were performed to predicate LMNB2 biological functions. The biological function of LMNB2 was estimated by MTT and flow cytometry assay. Additionally, western blot assay was used to examine protein expression levels.

Results: Increased LMNB2 expression was related with worsened cancer type-dependent survival. A relation between LMNB2 expression levels and immune cell infiltration was found. GO and KEGG enrichment analysis indicated that LMNB2 was involved in a series of pathways. Biology function assays revealed that down-regulation of LMNB2 impaired proliferation and cell cycle distribution. At the mechanical level, LMNB2 acts as a regulator of cyclinD1 and cyclinE1.

Conclusions: Altogether, these data suggest that LMNB2 may serve as a tumor promoter and could be a possible target for cancer therapy.