Research Paper Volume 16, Issue 11 pp 9680—9691
Daphnoretin inhibited SCI-induced inflammation and activation of NF-κB pathway in spinal dorsal horn
- 1 Department of Orthopaedics, Fuzhou Second General Hospital, School of Clinical Medicine, Fujian Medical University, Fuzhou 350007, China
- 2 Department of Orthopaedics, Fuzhou Second Hospital of Xiamen University, School of Medicine, Xiamen University, Fuzhou 350007, China
- 3 Fujian Provincial Clinical Medical Research Center for First Aid and Rehabilitation in Orthopaedic Trauma, Fuzhou Trauma Medical Center, Fuzhou 350007, China
Received: November 3, 2023 Accepted: April 16, 2024 Published: June 5, 2024
https://doi.org/10.18632/aging.205893How to Cite
Copyright: © 2024 Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective: Spinal cord injury (SCI) is a devastating disease for which there is no safe and effective treatment at present. Daphnoretin is a natural discoumarin compound isolated from Wikstroemia indica with various pharmacological activities. Our study aimed to investigate the role of Daphnoretin in NF-κB pathway activation and inflammatory response after SCI.
Methods: A mouse SCI model was constructed, and the Basso Mouse Scale Score and subscore were used to evaluate the effect of Daphnoretin on the movement capacity of mice. The effect of Daphnoretin on the activation of glial cells in the mouse model and BV2 cells was observed by immunofluorescence. PCR and ELISA were used to detect the expression of inflammatory factors, and Western blot was performed to detect the protein expression associated with NF-κB pathway.
Results: Daphnoretin inhibited the loss of movement ability and the activation of glial cells in mice after SCI, and it also inhibited the activation of NF-κB pathway and the expression of inflammatory factors TNF-α and IL-1β in vivo and in vitro.
Conclusions: Daphnoretin can inhibit the activation of NF-κB pathway and the inflammatory response induced by SCI. Our study demonstrates the potential of Daphnoretin on clinical application for the treatment of SCI.
Abbreviations
SCI: Spinal cord injury; NP: Neuropathic pain; BBB: Blood brain barrier; IL: Interleukin; TNF: Tumor necrosis factor; INF: Interferon; NF-κB: Nuclear factor-κB; IKK: IκB kinase; SPF: Specific-pathogen-free; LPS: Lipopolysaccharide; BMS: Basso Mouse Scale; DMEM: Dulbecco’s modified Eagle medium; FBS: Fetal bovine serum; PS: Penicillin-streptomycin; RT-qPCR: Real-Time quantitative PCR; CT: Cycle threshold; IF: Immunofluorescence; SDS-PAGE: Sodium dodecyl sulphate polyacrylamide gel electrophoresis; ROIs: Regions of interest; S.E.M: Standard error..