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Research Paper|Volume 15, Issue 12|pp 5535—5549

Galectin-1-mediated high NCAPG expression correlates with poor prognosis in gastric cancer

Tingrui Zheng1, Tao Qian2, Haihua Zhou1, Zhiyi Cheng1, Guiyuan Liu1, Chuanjiang Huang1, Rongrong Dou3, Fuxing Liu3, Xiaolan You1
  • 1Department of Gastrointestinal Surgery, The Affiliated Taizhou People’s Hospital of Nanjing Medical University, Taizhou 225300, Jiangsu, China
  • 2Department of Anesthesiology, The Affiliated Taizhou People’s Hospital of Nanjing Medical University, Taizhou 225300, Jiangsu, China
  • 3Department of the Pathology, The Affiliated Taizhou People’s Hospital of Nanjing Medical University, Taizhou 225300, Jiangsu, China
* Equal contribution
Received: March 27, 2023Accepted: May 24, 2023Published: June 16, 2023
https://doi.org/10.18632/aging.204806

Copyright: © 2023 Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Galectin-1 (Gal1) and non-SMC condensin I complex, subunit G (NCAPG) are associated with metastasis in several malignant tumors. However, their precise roles in gastric cancer (GC) remain uncertain. This study explored the clinical significance and relationship of Gal1 and NCAPG in GC. Gal1 and NCAPG expressions were significantly up-regulated in GC compared to adjacent non-cancerous tissues by immunohistochemistry (IHC) and Western blotting. Besides, methods including stable transfection, quantitative real-time reverse transcription PCR, Western blotting, Matrigel invasion and wound-healing assays in vitro, were also conducted. IHC scores for Gal1 and NCAPG had a positive correlation in GC tissues. High Gal1 or NCAPG expression significantly correlated with poor prognosis in GC, and Gal1 combined with NCAPG had a synergetic effect on the prediction of GC prognosis. Gal1 overexpression in vitro enhanced NCAPG expression, cell migration, and invasion in SGC-7901 and HGC-27 cells. Simultaneous Gal1 overexpression and NCAPG knockdown in GC cells partly rescued the migrative and invasive abilities. Thus, Gal1 promoted GC invasion through increased NCAPG expression. The present study demonstrated the prognostic significance of the combination of Gal1 and NCAPG in GC for the first time.