Research Paper Volume 15, Issue 10 pp 4108—4121
Association of NCAP family genes with prognosis and immune infiltration of human sarcoma
- 1 Department of Orthopedics, People’s Hospital of Pingchang County, Pingchang, Sichuan 636400, China
- 2 Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China
- 3 The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong 518000, China
- 4 Department of General Surgery, People’s Hospital of Pingchang County, Pingchang, Sichuan 636400, China
- 5 Department of Orthopedics, Longhui People’s Hospital, Shaoyang, Hunan 422200, China
- 6 Department of Orthopedics, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong 510900, China
Received: January 26, 2023 Accepted: April 17, 2023 Published: May 9, 2023
https://doi.org/10.18632/aging.204683How to Cite
Copyright: © 2023 Jiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective: This study was conducted to explore the correlation of NCAP family genes with expression, prognosis, and immune infiltration in human sarcoma.
Results: Compared with normal human tissues, six NCAP family genes were highly expressed in sarcoma tissues, and high expression of the six genes were significantly associated with the poor prognosis of sarcoma patients. The expression of NCAPs in sarcoma was significantly related to the low infiltration level of macrophages and CD4+ T cells. GO and KEGG enrichment analysis showed that NCAPs and their interacting genes were mainly enriched in organelle fission for biological processes (BP), spindle for cellular component (CC), tubulin binding for molecular function (MF), and ‘Cell cycle’ pathway.
Methods: We explored the expression of NCAP family members by ONCOMINE, and GEPIA databases. Additionally, the prognostic value of NCAP family genes in sarcoma was detected by Kaplan-Meier Plotter and GEPIA databases. Moreover, we explored the relationship between NCAP family gene expression level and immune infiltration using the TIMER database. Finally, we performed GO and KEGG analysis for NCAPs-related genes by DAVID database.
Conclusion: The six members of NCAP gene family can be used as biomarkers to predict the prognosis of sarcoma. They were also correlated with the low immune infiltration in sarcoma.
Abbreviations
AMPK: AMP-activated protein kinase; BC: Breast cancer; BP: Biological process; CC: Cellular component; CDK1: Cyclin-dependent kinase 1; DAVID: Database for Annotation, Visualization, and Integrated Discovery; DFS: Disease-free survival; E2F1: E2 promoter binding factor 1; FOXO4: Forkhead box subclass O protein 4; GEPIA: Gene Expression Profiling Interactive Analysis; GO: Gene ontology; HCC: Hepatocellular carcinoma; KEGG: Kyoto Encyclopedia of Genes and Genomes; MF: Molecular function; NCAPD2: non-SMC condensin I complex D2 subunit; NCAPD3: non-SMC condensin I complex D3 subunit; NCAPG: non-SMC condensin I complex G subunit; NCAPG2: non-SMC condensin I complex G2 subunit; NCAPH: non-SMC condensin I complex H subunit; NCAPH2: non-SMC condensin I complex H2 subunit; OS: Overall survival; qPCR: Quantitative PCR; RFS: relapse-free survival; SARC: Sarcoma; SMC: Structural maintenance of chromosomes; TIMER: Tumor Immune Estimation Resource.