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Research Paper|Volume 15, Issue 10|pp 4108—4121

Association of NCAP family genes with prognosis and immune infiltration of human sarcoma

Guangyao Jiang1,2, Qunyan Tian2, Peikai Shi3, Zhigao Li4, Yan Li1, Junjie Chen5, Wanchun Wang2, Ruiqi Chen2, Hua Zhong6, Gen Wu6
  • 1Department of Orthopedics, People’s Hospital of Pingchang County, Pingchang, Sichuan 636400, China
  • 2Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China
  • 3The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong 518000, China
  • 4Department of General Surgery, People’s Hospital of Pingchang County, Pingchang, Sichuan 636400, China
  • 5Department of Orthopedics, Longhui People’s Hospital, Shaoyang, Hunan 422200, China
  • 6Department of Orthopedics, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong 510900, China
* Equal contribution
Received: January 26, 2023Accepted: April 17, 2023Published: May 9, 2023
https://doi.org/10.18632/aging.204683

Copyright: © 2023 Jiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objective: This study was conducted to explore the correlation of NCAP family genes with expression, prognosis, and immune infiltration in human sarcoma.

Results: Compared with normal human tissues, six NCAP family genes were highly expressed in sarcoma tissues, and high expression of the six genes were significantly associated with the poor prognosis of sarcoma patients. The expression of NCAPs in sarcoma was significantly related to the low infiltration level of macrophages and CD4+ T cells. GO and KEGG enrichment analysis showed that NCAPs and their interacting genes were mainly enriched in organelle fission for biological processes (BP), spindle for cellular component (CC), tubulin binding for molecular function (MF), and ‘Cell cycle’ pathway.

Methods: We explored the expression of NCAP family members by ONCOMINE, and GEPIA databases. Additionally, the prognostic value of NCAP family genes in sarcoma was detected by Kaplan-Meier Plotter and GEPIA databases. Moreover, we explored the relationship between NCAP family gene expression level and immune infiltration using the TIMER database. Finally, we performed GO and KEGG analysis for NCAPs-related genes by DAVID database.

Conclusion: The six members of NCAP gene family can be used as biomarkers to predict the prognosis of sarcoma. They were also correlated with the low immune infiltration in sarcoma.