Research Paper|Volume 15, Issue 8|pp 2852—2862

A chronic wound model to investigate skin cellular senescence

Saranya P. Wyles¹, Parisa Dashti¹, Tamar Pirtskhalava², Burak Tekin³, Christina Inman², Lilian Sales Gomez⁴, Anthony B. Lagnado⁴, Larissa Prata², Diana Jurk², João F. Passos², Tamar Tchkonia², James L. Kirkland²

¹Department of Dermatology, Mayo Clinic, Rochester, MN 55905, USA
²Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA
³Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
⁴Department of Medicine, Division of General Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA

Received: December 28, 2022Accepted: April 3, 2023Published: April 21, 2023

https://doi.org/10.18632/aging.204667

Copyright: © 2023 Wyles et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Wound healing is an essential physiological process for restoring normal skin structure and function post-injury. The role of cellular senescence, an essentially irreversible cell cycle state in response to damaging stimuli, has emerged as a critical mechanism in wound remodeling. Transiently-induced senescence during tissue remodeling has been shown to be beneficial in the acute wound healing phase. In contrast, persistent senescence, as observed in chronic wounds, contributes to delayed closure. Herein we describe a chronic wound murine model and its cellular senescence profile, including the senescence-associated secretory phenotype.