Research Paper|Volume 14, Issue 12|pp 4959—4975

Time makes histone H3 modifications drift in mouse liver

Roman Hillje¹, Lucilla Luzi¹, Stefano Amatori², Giuseppe Persico¹, Francesca Casciaro⁴, Martina Rusin^1,², Mirco Fanelli², Piergiuseppe Pelicci^1,³, Marco Giorgio^1,⁴

¹Department of Experimental Oncology, IRCCS - European Institute of Oncology, Milano 20139, Italy
²Department of Biomolecular Sciences, Molecular Pathology Lab, University of Urbino 'Carlo Bo', Fano 61032, Italy
³Department of Oncology and Hemato-Oncology, University of Milan, Milano 20122, Italy
⁴Department of Biomedical Sciences, University of Padua, Padova 35131, Italy

Received: November 1, 2021Accepted: May 19, 2022Published: June 10, 2022

https://doi.org/10.18632/aging.204107

Copyright: © 2022 Hillje et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

To detect the epigenetic drift of time passing, we determined the genome-wide distributions of mono- and tri-methylated lysine 4 and acetylated and tri-methylated lysine 27 of histone H3 in the livers of healthy 3, 6 and 12 months old C57BL/6 mice. The comparison of different age profiles of histone H3 marks revealed global redistribution of histone H3 modifications with time, in particular in intergenic regions and near transcription start sites, as well as altered correlation between the profiles of different histone modifications. Moreover, feeding mice with caloric restriction diet, a treatment known to retard aging, reduced the extent of changes occurring during the first year of life in these genomic regions.