Aging
Navigate
Submit an Article
Research Paper|Volume 14, Issue 10|pp 4376—4389

CircRNA has_circ_0069313 induced OSCC immunity escape by miR-325-3p-Foxp3 axes in both OSCC cells and Treg cells

Yiyang Chen1, Zeyu Li2, Jianfeng Liang3, Jiayu Liu1, Jiansuo Hao1, Quan Wan1, Jiameng Liu1, Chongdai Luo1, Zhiyuan Lu1
  • 1Department of Oral and Maxillofacial Surgery, Stomatology Medical Center, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China
  • 2School of Stomatology of Qingdao University, Qingdao, China
  • 3Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, China
* Equal contribution
Received: January 3, 2022Accepted: April 25, 2022Published: May 16, 2022
https://doi.org/10.18632/aging.204068

Copyright: © 2022 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Introduction: CircRNAs are engaged in the tumorigenesis and progression of oral squamous cancer cells (OSCC). However, the function and underlying mechanism of circRNAs on tumor-associated immunity escape are largely unknown.

Materials and methods: We analyzed the expression pattern of has_circ_0069313 in our in-house database and its correlation with OSCC prognosis. Immunohistochemistry was applied to detected PDL1 expression. RNA fluorescence in situ hybridization was applied to detect subcellular location of circRNA. A luciferase activity assay was used to detect the interaction of has_circ_0069313 and miR-325-3p and its downstream target, Foxp3. Exosomes were collected to detect the exosomal circRNAs and co-culture assays were performed to detect the function of exosomal circRNAs on Tregs.

Results: has_circ_0069313 was upregulated in OSCC tissues and predicts poor prognosis. has_circ_0069313 promotes immunity escape through inhibiting miR-325-3p-induced Foxp3 degradation. has_circ_0069313 is an exosomal circRNA and the transfer of has_circ_0069313 to Treg cells promotes the Treg function through maintaining Foxp3 levels.

Conclusion: Our results indicate that has_circ_0069313 induces OSCC immunity escape via the miR-325-3p-Foxp3 axis in both OSCC cells and Treg cells.