Research Paper Volume 14, Issue 8 pp 3607—3616
Association of CIDEB gene promoter methylation with overweight or obesity in adults
- 1 College of Public Health, Zhengzhou University, Zhengzhou, Henan, China
- 2 Nursing Department of Jiaozuo People’s Hospital, Jiaozuo, Henan, China
- 3 Henan Huapu Pharmaceutical Technology Co., Ltd., Zhengzhou, Henan, China
- 4 School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China
Received: September 27, 2021 Accepted: March 25, 2022 Published: April 27, 2022
https://doi.org/10.18632/aging.204032How to Cite
Copyright: © 2022 Ping et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective: To explore the association of the methylation level of cell death-inducing DFF45-like effector B (CIDEB) gene promoter with overweight or obesity in the abdominal subcutaneous adipose tissue (SAT) and omental adipose tissue (OAT) of adults.
Methods: A total of 61 patients undergoing abdominal surgery in the hospital were selected with an average age of 51.87 years. According to the diagnostic criteria of Chinese adult obesity, the subjects were divided into normal-weight group (n = 28) and overweight/obesity group (n = 33). CIDEB promoter methylation level in abdominal SAT and OAT was detected by the MethylTarget technology, then its relationship with overweight or obesity was analyzed.
Results: (1) There were no statistical differences between the normal-weight group and overweight/obesity group in Methylation levels of 16 CpG sites in the CIDEB gene promoter sequence. (2) The methylation level of OAT was higher than that of SAT, and there were significant differences in 16 CpG sites. (3) There were 3 statistically significant haplotypes between the normal-weight group and overweight/obesity group (2 in SAT and 1 in OAT).
Conclusions: The methylation level of CIDEB gene promoter in abdominal SAT and OAT may be related to overweight or obesity in adults, and the specific regulatory mechanism needs to be further studied.
Abbreviations
BMI: body mass index; CIDEB: cell death-inducing DFF45-like effector B; FKBP5: FK506-binding protein 51 kDa; FPG: fasting plasma glucose; FSP27: fat-specific protein 27; HDL: high density lipoprotein; LDL: low density lipoprotein; OAT: omental adipose tissue; SAT: subcutaneous adipose tissue; TC: total cholesterol; TG: total triglyceride; VAT: visceral adipose tissue; VDR: vitamin D receptor; VLDL: very low density lipoprotein; WC: waist circumference.