Research Paper
Circular RNA RBM33 promotes breast cancer progression by targeting miR-17-5p/CBX7 signal pathway
- 1 Department of Breast and Nail Surgery, Xinjiang Medical University, Urumqi, Xinjiang, China
- 2 Burn Cosmetic Plastic Surgery Department, General Hospital of Pangang Group, Panzhihua, Sichuan, China
- 3 Department of Gynecology, Zhengzhou People’s Hospital, Zhengzhou, Henan Province, China
Received: May 4, 2021 Accepted: September 29, 2021
https://doi.org/10.18632/aging.How to Cite
Copyright: © 2022 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Purpose: There is increasing evidence of the vital role of circular RNAs (circRNAs) in breast cancer progression. A circRNA, hsa_ circ_ 0001772, was generated from the RBM33 gene and named circRBM33. The aim of this study was to investigate the role of circRBM33 in breast cancer.
Methods: qRT-PCR was performed to detect the expression of circRMB33. Ki-67 staining, clone formation, cell cycle assay was used to detect the proliferation ability in breast cancer cells. Transwell invasion assay was carried out for determining invasion ability. TUNEL assay was performed to assess the apoptosis level. Luciferase, AGO2 RIP, and pull-down assay were used to verify the relationship among circRBM33, miR-17a-5p, and CBX7.
Results: circRBM33 was found upregulated in breast cancer cell lines. Silencing of circRBM33 prevented proliferation and invasion ability in breast cancer cells. Knockdown of circRBM33 induced cell apoptosis. The effect of si-circRBM33 on breast cancer cells was blocked by Wnt signal activation. Bioinformatics databases predicted downstream targets of circRBM33 and miR-17a-5p. Luciferase reporter, AGO2 RIP, and pull-down assay performed the binding relationship between miR-17a-5p with circRBM33 and CBX7. In vivo, circRBM33 knockdown prevented tumor growth, which was abolished by CBX7.
Conclusion: CircRBM33 could promote breast cancer development via targeting the miR-17a-5p/CBX7. CircRBM33 could be used as a tumor biomarker and a possible therapeutic target in the future.