Research Paper
Silencing of long noncoding RNA MAFG-AS1 prevents autophagy and epithelial-mesenchymal transition via miR-24-3p/ B4GAT1 in ovarian cancer
- 1 Department of Obstetrics and Gynecology, Jinan Maternity and Child Care Hospital, Jinan, Shandong Province, China
Received: June 15, 2021 Accepted: September 2, 2021
https://doi.org/10.18632/aging.203565How to Cite
Copyright: © 2021 Yu and Li. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Ovarian cancer is one of the most common malignancies of the female reproductive system, with the second-highest incidence and the most lethality of female malignancies. Due to the lack of reliable biomarkers, 70% of ovarian cancer patients are already in advanced stages when diagnosed, while the 5-year survival rate is less than 50%. Therefore, it is a hot spot in ovarian cancer research to explore new diagnostic indicators and therapeutic targets assisted by early pathological molecular biology and to develop targeted drugs. LncRNA MAFG-AS1 promotes tumor growth in multiple cancers. However, the function of MAFG-AS1 in ovarian cancer has not been declared. Here, we explore the function and mechanism of MAFG-AS1 in ovarian cancer. MAFG-AS1 levels were upregulated in tumor tissues than adjacent normal tissues isolated from ovarian cancer patients. Silencing of MAFG-AS1 prevented cell proliferation, migration and invasion in ovarian cancer cells. Furthermore, we declared that MAFG-AS1 knockdown reduced autophagy, inhibited epithelial-mesenchymal transition in SKOV3 cells. MiR-24-3p, B4GAT1, the downstream target of MAFG-AS1 was verified by luciferase. Taken together, our research demonstrated the mechanism of MAFG-AS1 in ovarian cancer. MAFG-AS1 knockdown could prevent tumor development by inhibiting autophagy and the epithelial-mesenchymal transition pathway.