Research Paper Volume 16, Issue 11 pp 9485—9497
circRNA6448-14/miR-455-3p/OTUB2 axis stimulates glycolysis and stemness of esophageal squamous cell carcinoma
Figure 4. Stimulative effect on cell glycolysis and stemness by circRNA6448-14/miR-455-3p/OTUB2 axis. (A) qRT-PCR detection of OTUB2 expression in different treatment groups. (B) MTT detection of cell viability in different treatment groups. (C) Stem cell sphere formation assay in different treatment groups. (D, E) WB detection of stem cell surface marker proteins Nanog, OCT4, SOX2 expression and glycolysis rate-limiting enzymes HK2, LDHA expression in different treatment groups. (F, G) The ECAR and OCR of different treatment groups. (H–J) Glucose consumption, lactate production, and ATP generation levels in cells of different treatment groups. *P < 0.05.