Research Paper Volume 16, Issue 11 pp 9485—9497
circRNA6448-14/miR-455-3p/OTUB2 axis stimulates glycolysis and stemness of esophageal squamous cell carcinoma
Figure 3. Functional relationship of circRNA6448-14/miR-455-3p/OTUB2 axis. (A) qRT-PCR detection of miR-455-3p expression in normal esophageal epithelial cell HEEC and ESCC cells TE1, Eca109, KYSE150. (B, C) RIP assay and dual luciferase assay were used to verify the binding relationship between circRNA6448-14 and miR-455-3p. (D) Binding site of miR-455-3p and OTUB2. (E) Expression of OTUB2 in ESCC and normal tissues. (F, G) RIP assay and dual luciferase assay were used to verify the binding relationship between miR-455-3p and OTUB2. (H) qRT-PCR detection of OTUB2 expression in normal esophageal epithelial cells HEEC and ESCC cells TE1, Eca109, KYSE150. (I) qRT-PCR detection of OTUB2 expression in different treatment groups. *P < 0.05.