Research Paper Volume 16, Issue 11 pp 9485—9497

Figure 2. circRNA6448-14 increases stemness and glycolysis in ESCC cells. (A) qRT-PCR detection of circRNA6448-14 expression in circRNA6448-14 knockdown and control cells. (B) MTT assay detection of cell viability in circRNA6448-14 knockdown and control cells. (C) Stem cell sphere formation experiment in circRNA6448-14 knockdown and control cells. (D, E) WB detection of stem cell surface marker proteins Nanog, OCT4, SOX2 expression and glycolysis rate-limiting enzymes HK2, LDHA expression in circRNA6448-14 knockdown and control cells. (F) The ECAR of circRNA6448-14 knockdown and control cells. (G) The OCR of circRNA6448-14 knockdown and control cells. (H–J) The kits detected glucose consumption, lactate production, and ATP generation levels in cells. ^*P < 0.05.