Research Paper Volume 16, Issue 4 pp 3955—3972

Figure 4. Activation of the PI3K/Akt pathway was involved in the protective effect of triptolide on ARPE-19 cells. (A) Serum Nrf2 levels in normal subjects and AMD patients detected by ELISA, n = 3. (B) Cells were treated with 20 nM triptolide (TP) for 6 h and then treated with sodium iodate (SI) for 24 h. Western blot analysis was performed using the corresponding antibodies. Quantitative analyses of p-PI3K/PI3K (C), p-Akt/Akt (D), p-Nrf2/Nrf2 (E), HO-1 (F) and NQO1 (G) by ImageJ software. Data are shown as mean ± standard deviation (SD) (n = 3); Abbreviation: NS: not significant. ^*p < 0.05. ^**P < 0.01.