Research Paper Volume 16, Issue 4 pp 3583—3595
Rutin promotes osteogenic differentiation of mesenchymal stem cells (MSCs) by increasing ECM deposition and inhibiting p53 expression
Figure 1. The effect of rutin on MSC osteogenesis and differentiation. (A, B) Western blot showed that rutin treatment in MSC culture improved the early and late osteogenic markers (OCN, OPN, RUNX2) level. (C) The AR staining. (D) Real-time RT-PCR analysis showed that the expression of early and late osteogenic markers (ALP, OCN, OPN, RUNX2) was significantly enhanced by rutin treatment. (E) Real-time RT-PCR analysis shows that the expression of adipogenic markers (Pparg, Fabp4) was significantly inhibited by treatment with rutin. The quantitative data were expressed as the mean ± S.D. of three independent experiments (*p < 0.05).