Research Paper Volume 15, Issue 23 pp 14172—14191

Figure 6. Effect of GP on neuronal apoptosis in the hippocampus of STP Rats. (A) Representative TUNEL-positive cells (green) in hippocampal CA3 region. Nuclei were stained with DAPI (in blue). Scale bar = 200 μm. (B) Quantification of the TUNEL/DAPI using ImageJ software. (C–F) Representative images and quantitative analysis of Western blotting for Bcl-2, Bax, and caspase-3 in the hippocampus after normalization to the β-actin signal. Data represent mean ± SEM, p-value significance is calculated from one-way ANOVA, n = 3. ^*p < 0.05, ^**p < 0.01, ^***P < 0.001 and ^****P < 0.0001.