Research Paper Volume 15, Issue 14 pp 6757—6773

Figure 6. Loss of PARP10 impaired proliferation of AML cells. (A) Sanger sequencing showing random insertions and/or deletions introduced by CRISPR/Cas9-mediated PARP10 editing in MOLM13 cells. (B) Flow cytometry-based RFP competition assay showing PARP10 knockout by two sgRNAs impaired cell proliferation in MOLM13 cells. Proliferation curves of PARP10 knockout cells introduced by sgRNA1 (C) and sgRNA2 (D) versus control MOLM13 cells, the error bars represent the SD from triplicates. The asterisk indicates significant statistical differences between the PARP10 knockout and control cells.