Research Paper Volume 15, Issue 9 pp 3356—3380

Figure 5. Comparison of age-related changes to the immune microenvironment of the mouse bladder, kidneys, and prostate. (A) Principal component analysis (PCA) was performed on immune cell cluster frequencies for mouse prostates, bladders, and kidneys at 3- and 16-months-old. Ellipses represent 95% confidence intervals for 4 biological replicates at each age and tissue. Abbreviation: PC: principal component. (B) Heat map showing correlations with age (ρ) for immune cell clusters from mouse prostate, bladder, and kidneys irrespective of p-value. Shading represents Spearman correlation (ρ). (C–E) Quantification of immune cell cluster abundance at 3 and 16 months of age in mouse prostate, bladder, and kidney separated into T cells (C), B cells (D), and myeloid cells (E). Mann Whitney U test between 3- and 16-months-old, ^*p < 0.05. Data represents mean ± SD of 4 biological replicates at each age and tissue.