Research Paper Volume 12, Issue 12 pp 11337—11348

Senescence markers in DIO-mice after senolytic treatment. (A) Macroscopic view of perigonadal white adipose tissue (pWAT) from mice treated with the 5 cycles of senolytics and stained for senescence-associated β-Galactosidase (SA-β-Gal) activity. V: vehicle. Nav: navitoclax. D/Q:dasatinib/quercetin. (B) Microscopic images of the same pWATs shown in (A). (C) Quantification of the blue area, positive for SA-βGal, of 6 fields per condition from the pWATs shown in (A) and (B). (D) mRNA expression analysis of the indicated genes in pWAT obtained at the day of sacrifice. Statistical significance was assessed using the one-way ANOVA test with Tukey's correction for multiple comparisons (C) or the two-tailed unpaired Student's t-test between each treatment and its control (D) Asterisks refer to the comparison with the corresponding vehicle-treated mice. *, p<0.05; **, p<0.01.