Research Paper Volume 12, Issue 12 pp 12305—12323
Inhibition of BMP3 increases the inflammatory response of fibroblast-like synoviocytes in rheumatoid arthritis
Figure 5. Overexpression of BMP3 decreases proinflammatory cytokines and chemokines in RA and AIA FLS. (A) The protein levels of BMP3, IL-6, IL-1β, and IL-17A in TNF-α–treated RA FLS transfected with BMP3-pcDNA3.1 were analyzed using western blot. (B) The protein levels of BMP3, IL-6, IL-1β, and IL-17A in TNF-α–treated AIA FLS transfected with BMP3-PEX were analyzed using western blot. (C) The mRNA levels of BMP3, IL-6, IL-1β, and IL-17A in TNF-α–treated RA FLS transfected with BMP3-pcDNA3.1 were analyzed using qPCR. (D) The mRNA levels of BMP3, IL-6, IL-1β, and IL-17A in TNF-α–treated AIA FLS transfected with BMP3-PEX were analyzed using qPCR. (E) The mRNA levels of CCL2, CCL3, and VCAM-1 in TNF-α–treated RA FLS transfected with BMP3-pcDNA3.1 were analyzed using qPCR. (F) The mRNA levels of CCL2, CCL3, and VCAM-1 in TNF-α–treated AIA FLS transfected with BMP3-PEX were analyzed using qPCR. All values are expressed as the mean ± SD. *P < 0.05, **P < 0.01 vs AIA group. #P < 0.05, ##P < 0.01 vs NC-PEX group.