Research Paper Volume 8, Issue 10 pp 2568—2589

Figure 1. The long-lived mutant strains 3, 5 and 12 do not differ from the parental WT strain in the exponential growth rates and post-exponential growth efficacies in medium initially containing fermentable or non-fermentable carbon source. The parental haploid WT strain BY4742, long-lived mutant strains 3, 5 and 12 (each in the BY4742 genetic background), and the single-gene-deletion mutant strains rpp2BΔ and dbp3Δ (each in the BY4742 genetic background) were cultured in YP medium initially containing 0.2% glucose (A), 2.0% glucose (B), 1.0% ethanol (C) or 3.0% glycerol (D). The OD₆₀₀ of cell cultures recovered at different time points was measured. Growth curves are shown; data for growth curves are presented as means (n = 3). For each strain, a doubling time (min) was calculated as T_d = (t₂ – t₁) × log 2/log (OD₂/OD₁), where: t₂ = a given time point; t₁ = an earlier time point; OD₂ = OD₆₀₀ at a given time point; OD₁ = OD₆₀₀ at an earlier time point. Data for the values of T_d are presented as means ± SEM (n = 3; ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001).