Research Paper Volume 4, Issue 9 pp 636—647
Down regulation of miR-124 in both Werner syndrome DNA helicase mutant mice and mutant Caenorhabditis elegans wrn-1 reveals the importance of this microRNA in accelerated aging
Figure 5. Impact of vitamin C on the life span of wild type and mutant C. elegans strains. (A) Survival curves of vitamin C treated wrn-1(gk99) and of untreated wild-type (N2) and wrn-1(gk99) C. elegans strains grown at 25°C (vitamin C treated wrn-1(gk99) vs. untreated wrn-1(gk99) worms: P = 1.4 × 10−7; vitamin C treated wrn-1(gk99) vs. untreated wild-type worms: P = 0.0778). (B) Survival curves of vitamin C treated mir-124(n4255) and of untreated wild-type (N2) and mir-124(n4255)C. elegans strains grown at 25°C (vitamin C treated mir-124(n4255) vs. untreated mir-124(n4255) worms: P = 3.0 × 10−9; vitamin C treated wrn-1(gk99) vs. untreated wild-type worms: P = 0.359). (C) Survival curves of vitamin C treated wrn-1;mir-124 and of untreated wild-type (N2) and wrn-1;mir-124 double mutant C. elegans strains grown at 25°C (vitamin C treated wrn-1;mir-124 vs. untreated wrn-1;mir-124 worms: P = 3.1 × 10−6; vitamin C treated wrn-1;mir-124 vs. untreated wild-type worms: P = 0.0163). All experiments were performed three to four times with 20 to 30 worms per genotype. P-values were obtained using the log-rank test method.