Research Paper Volume 3, Issue 11 pp 1078—1091
Yeast-like chronological senescence in mammalian cells: phenomenon, mechanism and pharmacological suppression
Figure 7. Lactate resistance of HT-p21-9, HCT116 and HCT116-Bax−/− cells. HCT116 cells, HCT-Bax−/− and HT-p21-9 cells were plated in 96-well plates at low density (2000 cells/well) and treated with a range of lactic acid (LA) concentrations or conditioned medium (CM). After 3 days, medium was changed to fresh medium and cells were allowed to proliferate for 5 days and then numbers of live cells were determined using MTT assay. CM and R-CM were prepared as described in Methods.