Research Paper Volume 16, Issue 21 pp 13371—13391
Upregulation of multiple key molecules is correlated with poor prognosis and immune infiltrates in hepatocellular carcinoma by bulk and single-cell RNA-seq
- 1 Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- 2 Department of Infectious Diseases, Xingtai People’s Hospital, Xingtai, China
- 3 Department of Gastroenterology, Nanyang Second General Hospital, Nanyang, China
- 4 Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China
Received: February 1, 2024 Accepted: September 12, 2024 Published: November 12, 2024
https://doi.org/10.18632/aging.206151How to Cite
Copyright: © 2024 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: Recent discoveries in hepatocellular carcinoma (HCC) unveil key molecules. However, due to liver cancer’s high heterogeneity, predicting patient prognosis is challenging. This study aims to construct a model for predicting HCC prognosis using multiple key genes.
Methods: TCGA provided RNA expression and clinical data, differentially analyzed by DESeq2, edgeR, and Limma. The hub gene was pinpointed via CytoHubba’s degree algorithm in Cytoscape. GO and KEGG analyses illuminated potential pathways. Single-cell sequencing detailed key gene expression in diverse cell types. The LASSO regression model predicted patient prognosis.
Result: In the RNA-seq analysis using three R packages, we identified 762 differentially expressed genes, with Cytoscape revealing ten key genes showing significant prognostic value (P < 0.05). GO and KEGG analyses highlighted key biological processes and pathways. IHC confirmed higher expression in cancer tissues. Reduced immune cell infiltration was observed in HCC tissues, and immune checkpoint analysis showed a strong correlation between PD1, CTLA4, and hub genes. Single-cell sequencing indicated higher expression of key genes in immune cells than hepatocytes. Cox analysis validated the riskScore as a reliable, independent prognostic marker (HR = 4.498, 95% CI: 2.526–8.007).
Conclusions: The results from differential analysis using three R packages are robust, revealing genes closely linked to immune cell infiltration in the tumor microenvironment. Additionally, a validated prognostic model for liver cancer was established based on key genes.