Research Paper Advance Articles
Effectiveness of vaginal probiotics Lactobacillus crispatus chen-01 in women with high-risk HPV infection: a prospective controlled pilot study
- 1 Department of Gynaecology, The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006, People’s Republic of China
- 2 Department of Gynaecology, The First Hospital of Nanchang, Nanchang, Jiangxi 330006, People’s Republic of China
- 3 Queen Mary School, Nanchang University, Nanchang 330031, People’s Republic of China
- 4 Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China
- 5 Department of Gynaecology, Jingdezhen Maternal and Child Health Care Hospital, Jingdezhen, Jiangxi 333000, People’s Republic of China
- 6 School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, People’s Republic of China
- 7 National Engineering Research Center for Bioengineering Drugs and The Technologies, Institution of Translational Medicine, Jiangxi Medical College, Nanchang University, Nanchang, People’s Republic of China
Received: December 21, 2023 Accepted: July 15, 2024 Published: July 25, 2024
https://doi.org/10.18632/aging.206032How to Cite
Copyright: © 2024 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Female genital tract infection with high-risk human papilloma virus (HR-HPV) has the risk of developing into cervical cancer, and there is still a lack of effective therapeutic strategies. Probiotic intervention is considered as a potential intervention for HR-HPV, while exploration into living probiotic preparations for specific diseases remains limited and insufficient. This prospective controlled pilot study was conducted to observe the effect of intravaginal transplantation of a vaginal isolated natural probiotic strain, Lactobacillus crispatus chen-01, on the clearance of high-risk HPV infection. 100 women with high-risk HPV infection were enrolled and randomly divided into placebo group and probiotic treatment group, which received intravaginal transplantation of L. crispatus chen-01. Cervical exfoliated cells were collected 6 months later for detecting DNA load, typing of HPV, and cytological analysis. Our results showed that vaginal transplantation with L. crispatus chen-01 significantly reduced viral load of HPV, ameliorated HPV clearance rate, and improved vaginal inflammation state without causing obvious adverse reactions. Analysis of 16S rRNA sequencing revealed that L. crispatus chen-01 could effectively reconstitute the vaginal microbiota in women with high-risk HPV, which might be one of the underlying mechanisms of the beneficial effect of L. crispatus chen-01 transplantation. Our results suggested that vaginal transplantation of L. crispatus chen-01 might be a promising treatment for patients with high-risk HPV infection.