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Research Paper|Volume 15, Issue 11|pp 4746—4756

Calcium silicate-stimulated adipose-derived stem cells promote angiogenesis and improve skin wound healing

Mingming Wang1,3, Hongyan Zhan2, Jianhua Wang1,3, Hua Song3, Jianhua Sun3, Gang Zhao1
  • 1Department of Orthopaedics, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China
  • 2Department of B-Ultrasound, The Fourth People’s Hospital of Jinan, Jinan, Shandong, China
  • 3Department of Orthopaedics, Tengzhou Central People’s Hospital, Tengzhou, Shandong, China
Received: February 28, 2023Accepted: May 9, 2023Published: June 1, 2023
https://doi.org/10.18632/aging.204760

Copyright: © 2023 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Skin wound healing is a complicated process involving proliferation, inflammation, coagulation, and hemostasis, and scar tissue formation of wound repairing. Adipose-derived stem cells (ADSCs) have presented potential therapeutic effects in the non-healing and chronic wound. Calcium silicate (CS) ceramics have been identified as a new type of bioceramics for tissue construction and regeneration. Here, we aimed to explore the impact of CS on the regulation of ADSCs-mediated wound healing. Significantly, CS was able to dose-dependently enhance the proliferation of ADSCs. CS inhibited terminal deoxynucleotidyl transferase dUTP nick end labeling positive cells in the H2O2-treated ADSCs. Similarly, the Bcl-2 expression was elevated while Bax and cleaved caspase-3 expression were repressed by CS in the cells. CS could induce migration and reduce oxidative stress of ADSCs. Moreover, immunofluorescence analysis and Western blot analysis showed that CS could promote CXCR4 expression in ADSCs. Moreover, CS-stimulated ADSCs enhanced migration and angiogenic capacity of HUVEC. Importantly, CS-stimulated ADSCs improved wound healing in full-thickness skin defect mouse model. Thus, we conclude that CS improves ADSCs-attenuated wound healing in vivo and in vitro. Our finding presents novel insight in the scenario that CS regulates ADSCs and wound healing. CS may be applied as potential materials for the treatment of wound healing.