Research Paper|Volume 15, Issue 6|pp 1918—1930

Haplotype-GGGT in long non-coding RNA MALAT1 inhibits brain metastatic lung cancer and lymph nodes of lung cancer via the MALAT1/miR-328/KATNB1

Tingting Liu¹, Jianpeng Ma², Dongmei Hou¹, Weiqi Wang³, Hetao Cao¹

¹Department of Medical Imaging, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China
²Department of Magnetic Resonance Imaging, Dingbian County People’s Hospital, Dingbian, Yulin, Shaanxi 718600, China
³School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China

* Equal contribution

Received: October 29, 2022Accepted: February 15, 2023Published: March 2, 2023

https://doi.org/10.18632/aging.204563

Copyright: © 2023 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The up-regulation of katanin P80 has been reported to be correlated with a larger tumor size and lymph node metastasis in non–small-cell lung cancer (NSCLC) patients. And lncRNA MALAT1 was demonstrated to promote the proliferation of chronic myeloid leukemia cells via modulating miR-328. 135 lung cancer patients were divided into 6 groups according to their genotypes of MALAT1. The expression of KATNB1 was negatively correlated with the GGGT genotype of MALAT1. Decreased lymph node size and tumor size of brain metastatic lung were observed in patients with GGGT genotype of MALAT1. The luciferase activities of MALAT1 and KATNB1 were remarkably suppressed by miR-328 in A549 and H460. And the down-regulation of MALAT1 or up-regulation of miR-328 significantly repressed the KATNB1 expression in A549 and H460 cells. MALAT1 expression was reduced in patients carrying haplotype GGGT. A signaling pathway of MALAT1/miR-328/KATNB1 was established to explain the down-regulation of KATNB1 mRNA in patients carrying haplotype GGGT and reduced lymph node size in lung cancer and tumor size in brain metastatic lung cancer.