Research Paper Advance Articles
An active ingredient isolated from Ganoderma lucidum promotes burn wound healing via TRPV1/SMAD signaling
- 1 Guangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou 510663, P. R. China
- 2 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, P. R. China
- 3 Guangdong Yuewei Bioscience Co., Ltd., Zhaoqing 526000, P. R. China
- 4 Sunnybrook Research Institute, Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto ON M5S 1A8, Canada
Received: March 11, 2022 Accepted: May 13, 2022 Published: June 13, 2022https://doi.org/10.18632/aging.204119
How to Cite
Copyright: © 2022 Jiao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The mushroom Ganoderma lucidum is a traditional Chinese medicine and G. lucidum spore oil (GLSO) is the lipid fraction isolated from Ganoderma spores. We examined the effect of GLSO on burn wound healing in mice. Following wounding, GLSO was applied on the wounds twice daily. Repair analysis was performed by Sirius-Red-staining at different time points. Cell proliferation and migration assays were performed to verify the effect of GLSO on growth. Network pharmacology analysis to identify possible targets was also carried out, followed by Western blotting, nuclear translocation, cell proliferation, and immunofluorescence assays for in-depth investigation of the mechanism. Our study showed that GLSO significantly promoted cell proliferation, and network pharmacology analysis suggested that GLSO might act through transient receptor potential vanilloid receptor 1 (TRPV1)/SMAD signaling. Furthermore, GLSO elevated SMAD2/3 expression in skin burn and promoted its nuclear translocation, and TRPV1 expression was also increased upon exposure to GLSO. Cell proliferation and immunofluorescence assays with TRPV1 inhibitor showed that GLSO accelerated skin burn wound healing through TRPV1 and SMADs signaling, which provides a foundation for clinical application of GLSO in the healing of deep skin burns.
GLSO: G. lucidum spore oil; TRPV1: Transient receptor potential vanilloid receptor 1; TCM: Traditional Chinese medicine; GMP: Good manufacturing practice; HPLC: High performance liquid chromatography; PBS: Phosphate-buffered saline; SMILES: Simplified molecular input line entry specification; OMIM: Online Mendelian Inheritance in Man; PPI: Protein-protein interaction; KEGG: Kyoto Encyclopedia of Genes and Genomes; RIPA: Radio-immunoprecipitation Assay; SDS-PAGE: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; CNR1: Cannabinoid receptor 1; PTGS2: Prostaglandin-endoperoxide synthase 2; NOS2: Nitric oxide synthase 2; TGF: Transforming growth factor.