Research Paper
FCGR2A as one novel potential target for poor survival prognosis of clear cell renal cell carcinoma
- 1 Department of Urology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China
Received: December 28, 2021 Accepted: April 25, 2022
https://doi.org/10.18632/aging.204049How to Cite
Copyright: © 2022 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: Clear cell renal cell carcinoma (ccRCC) is the most common type of renal cell carcinoma (RCC). Immunoglobulin Fc receptor FCGR2A has been implicated in various cancers, however, its role on ccRCC is not well studied.
Methods: A total of 151 patients with ccRCC were recruited for the study. Cox proportional hazards regression analysis was performed to calculate the hazard radios of FCGR2A expression and tumor characteristics. Pathological changes associated with ccRCC in tumor tissue sections were analyzed by hematoxylin-eosin staining. Immunohistochemical and immunofluorescence staining were used to detect the protein expression of FCGR2A in the tissue sections. Correlation between the expression of FCGR2A and the overall survival (OS) of ccRCC patients was analyzed by BP neural network and support vector machine (SVM).
Results: The expression of FCGR2A was significantly correlated with the pathological grade of tumor, family history of ccRCC and Enneking stage of ccRCC. Patients with high FCGR2A expression in the tumor tissue, had poorer OS than the patients with low and moderate FCGR2A expression. The ROC curve showed that FCGR2A can be used as a sensitive and specific biomarker for the diagnosis of ccRCC. Western blotting revealed that the FCGR2A was expressed at higher levels in the ccRCC tissues. BP neural network and SVM fitting showed that the R2 between FCGR2A and survival time of ccRCC patients was 0.8429 and 0.7669, respectively.
Conclusions: FCGR2A is highly expressed in ccRCC, higher expression of FCGR2A is associated with poorer OS of ccRCC.
Abbreviations
ccRCC: Clear cell renal cell carcinoma; RCC: Renal Cell Carcinoma; SVM: support vector machine; FcγRs: Immunoglobulin IgGFc receptor; HRs: hazard ratios; 95%CI: 95% confidence intervals; OS: overall survival; AUC: area under the curve; FCGR2A: FcγRIIa receptor; ADCP: antibody dependent cell phagocytosis.