Research Paper Volume 13, Issue 23 pp 25342—25364
Protective effects of galangin against H2O2/UVB-induced dermal fibroblast collagen degradation via hsa-microRNA-4535-mediated TGFβ/Smad signaling
- 1 Department of Plastic and Reconstructive Surgery, China Medical University Hospital, Taichung 40447, Taiwan, ROC
- 2 School of Medicine, China Medical University, Taichung 40447, Taiwan, ROC
- 3 Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung 406, Taiwan, ROC
- 4 Ph.D. Program for Biotechnology Industry, China Medical University, Taichung 406, Taiwan, ROC
- 5 China Medical University Beigang Hospital Thoracic Department, Yunlin 651, Taiwan, ROC
- 6 Division of Breast Surgery, Department of Surgery, China Medical University Hospital, Taichung 40447, Taiwan, ROC
- 7 Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
- 8 Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan, ROC
- 9 Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien 970, Taiwan, ROC
- 10 Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan, ROC
- 11 Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan, ROC
- 12 Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung 413, Taiwan, ROC
Received: July 19, 2021 Accepted: November 23, 2021 Published: December 10, 2021
https://doi.org/10.18632/aging.203750How to Cite
Copyright: © 2021 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
This study aimed to investigate the mechanism underlying the protective effects of galangin against H2O2/UVB-induced damage using in vitro and in vivo models of photodamage. Moreover, we identified the involvement of miRNA regulation in this process. The H2O2/UVB-treated HS68 human dermal fibroblasts and UVB-induced C57BL/6J nude mice were used as in vitro and in vivo models of photodamage. The results showed that galangin treatment alleviated H2O2/UVB-induced reduction in cell viability, TGFβ/Smad signaling impairment, and dermal aging. Based on the results of microRNA array analyses and database searches, hsa-miR-4535 was identified as a potential candidate miRNA that targets Smad4. In vitro, galangin treatment activated Smad2/3/4 complex and inhibited hsa-miR-4535 expression in H2O2/UVB-exposed cells. In vivo, topical application of low (12 mg/kg) and high doses (24 mg/kg) of galangin to the dorsal skin of C57BL/6J nude mice significantly alleviated UVB-induced skin photodamage by promoting TGFβ/Smad collagen synthesis signaling, reducing epidermal hyperplasia, wrinkle formation, and skin senescence, as well as inhibiting hsa-miR-4535 expression. Taken together, our findings indicate a link between hsa-miR-4535 and TGFβ/Smad collagen synthesis signaling and suggest these factors to be involved in the photo-protective mechanism of galangin in dermal fibroblasts against H2O2/UVB-induced aging. The evidence indicated that galangin with anti-aging properties can be considered as a supplement in skin care products.