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Research Paper|Volume 13, Issue 8|pp 11833—11859

Transcriptome integration analysis and specific diagnosis model construction for Hodgkin's lymphoma, diffuse large B-cell lymphoma, and mantle cell lymphoma

Wen-Xing Li1,2, Shao-Xing Dai3, San-Qi An4, Tingting Sun5, Justin Liu6, Jun Wang7, Leyna G. Liu8, Yang Xun7, Hua Yang7, Li-Xia Fan7, Xiao-Li Zhang7, Wan-Qin Liao7, Hua You9, Luca Tamagnone10, Fang Liu7, Jing-Fei Huang11, Dahai Liu7
  • 1Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China
  • 2Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Southern Medical University, Guangzhou, Guangdong, China
  • 3Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China
  • 4Biosafety Level-3 Laboratory, Life Sciences Institute & Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Collaborative Innovation Center for Biomedicine, Guangxi Medical University, Nanning, Guangxi, China
  • 5National School of Development, Peking University, Beijing 100871, China
  • 6Department of Statistics, University of California, Riverside, CA 92521, USA
  • 7Foshan Stomatology Hospital, School of Medicine, Foshan University, Foshan, Guangdong, China
  • 8Portola High School, Irvine, CA 92618, USA
  • 9Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, Guangdong, China
  • 10Istituto di Istologia ed Embriologia, Università Cattolica del Sacro Cuore, Rome, Italy
  • 11Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China
* Equal contribution
# Lead contact
Received: September 18, 2020Accepted: March 2, 2021Published: April 22, 2021

Copyright: © 2021 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Transcriptome differences between Hodgkin's lymphoma (HL), diffuse large B-cell lymphoma (DLBCL), and mantle cell lymphoma (MCL), which are all derived from B cell, remained unclear. This study aimed to construct lymphoma-specific diagnostic models by screening lymphoma marker genes. Transcriptome data of HL, DLBCL, and MCL were obtained from public databases. Lymphoma marker genes were screened by comparing cases and controls as well as the intergroup differences among lymphomas. A total of 9 HL marker genes, 7 DLBCL marker genes, and 4 MCL marker genes were screened in this study. Most HL marker genes were upregulated, whereas DLBCL and MCL marker genes were downregulated compared to controls. The optimal HL-specific diagnostic model contains one marker gene (MYH2) with an AUC of 0.901. The optimal DLBCL-specific diagnostic model contains 7 marker genes (LIPF, CCDC144B, PRO2964, PHF1, SFTPA2, NTS, and HP) with an AUC of 0.951. The optimal MCL-specific diagnostic model contains 3 marker genes (IGLV3-19, IGKV4-1, and PRB3) with an AUC of 0.843. The present study reveals the transcriptome data-based differences between HL, DLBCL, and MCL, when combined with other clinical markers, may help the clinical diagnosis and prognosis.