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Research Paper|Volume 13, Issue 2|pp 2941—2958

Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2

Baohua Zhang1, Haiyan Cui2, Yinping Sun3, Xinmei Wang1, Qing Jia4, Jing Li1, Yingchun Yin1, Xiaoyu Sun1, Huirong Xu1, Hongwei Li1, Famei Xu1, Jiansheng Rong1
  • 1Department of Pathology, Zibo Central Hospital, Zibo 255000, Shandong Province, China
  • 2Department of Pathology, Zibo Fourth People’s Hospital, Zibo 255067, Shandong Province, China
  • 3The Third Ward of Oncology Department, Zibo Central Hospital, Zibo 255000, Shandong Province, China
  • 4The First Ward of Gastroenterology Department, Zibo Central Hospital, Zibo 255000, Shandong Province, China
Received: July 24, 2020Accepted: December 3, 2020Published: January 13, 2021
https://doi.org/10.18632/aging.202444

Copyright: © 2021 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Gallbladder carcinoma (GC) is an extremely malignant gastrointestinal tumor, but relevant mechanisms are still under investigation. MicroRNA (miR) is differentially expressed in a variety of tumors. Here we explored miR-204 in patients with GC and related mechanisms. A GSE104165 chip was downloaded from the gene expression omnibus (GEO) for analysis. The qRT-PCR assay was used for quantifying miR-204 and Notch2 in the serum and tissues of the patients, and the patients were followed up for 3 years to analyze independent factors of prognosis. The CCK8, transwell, and flow cytometry assays were applied for analyzing proliferation, invasion, as well as apoptosis of cells, and the dual luciferase reporter (DLR) assay was adopted for determining the association of miR-204 with Notch2. MiR-204 was low in patients with GC, and it might serve as a diagnostic indicator for GC. In addition, patients with low e MiR-204 usually faced high rates of III+IV stage, distant metastasis, and low differentiation, and also showed a poor prognosis. DLR assay verified the targeted binding of miR-204 to Notch2 mRNA.