Research Paper|Volume 13, Issue 2|pp 2668—2680

Notch-1 promotes the malignant progression of osteosarcoma through the activation of cell division cycle 20

Yuan Gao², Lunhao Bai¹, Guanning Shang¹

¹Department of Orthopedic Surgery, ShengJing Hospital, China Medical University, Liaoning, PR China
²Graduate School, China Medical University, Shenyang, Liaoning Province, PR China

Received: September 2, 2020Accepted: October 22, 2020Published: December 19, 2020

https://doi.org/10.18632/aging.202314

Copyright: © 2020 Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The molecular mechanism of osteosarcoma (OS) pathogenesis is poorly understood. The Notch signaling pathway has been shown to be critically involved in tumorigenesis, including OS. Therefore, we explored the molecular mechanism by which the Notch-1 signaling pathway is involved in OS progression. Several approaches were carried out to determine the biological function of Notch-1 in OS cells. The MTT results revealed that Notch-1 overexpression increased the viability of OS cells, whereas Notch-1 downregulation reduced cell viability. Consistently, modulation of Notch-1 regulated apoptosis and the migratory and invasive abilities of OS cells. Mechanistic studies showed that Notch-1 overexpression augmented cell division cycle 20 (Cdc20) expression in OS cells. Moreover, overexpression of Cdc20 alleviated the inhibitory effects of Notch-1 downregulation on the viability, migration and invasion of OS cells. Our study offers a promising OS treatment strategy by inhibiting Notch-1.