Research Paper Volume 12, Issue 14 pp 14885—14896
Circular RNA DHX33 promotes malignant behavior in ccRCC by targeting miR-489-3p/MEK1 axis
- 1 Department of Urology, Ninghai First Hospital, Zhejiang 315600, China
- 2 Department of Urology, Ninghai Hospital, Branch of Shanghai Tenth People’s Hospital, Zhejiang 315600, China
- 3 Department of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, China
- 4 Department of Anesthesia, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China
- 5 Department of Urology, Shanghai Tenth People’s Hospital, Tongji University, Shanghai 200072, China
Received: March 11, 2020 Accepted: June 4, 2020 Published: July 27, 2020
https://doi.org/10.18632/aging.103550How to Cite
Abstract
Mounting evidence indicates that circular RNAs modulate the initiation of clear cell renal cell carcinoma (ccRCC). However, their specific roles in the malignancy of ccRCC is understudied. Here, we present a novel circular RNA, circDHX33, that is up-regulated in ccRCC cell lines and tissues. Upregulated circDHX33 in ccRCC patients significantly correlates with advanced TNM stage and metastasis. Suppressing circDHX33 expression inhibits the proliferation and invasion of cultured cells, and suppresses tumor growth in vivo. Mechanistically, we show that circDHX33 promotes ccRCC progression by sponging miR-489-3p and modulating MEK1 expression. In conclusion, our findings suggest that circDHX33 plays a role in promoting ccRCC via the miR-489-3p/MEK1 axis and may serve as a novel therapeutic target for the treatment of ccRCC patients