Research Paper Volume 12, Issue 11 pp 10398—10414
Testosterone enhances mitochondrial complex V function in the substantia nigra of aged male rats
- 1 Department of Neurobiology, Hebei Medical University, Shijiazhuang 050017, China
- 2 Department of Sports Medicine, Hebei Sport University, Shijiazhuang 050017, China
- 3 Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, China
- 4 Neuroscience Research Center, Hebei Medical University, Shijiazhuang 050017, China
- 5 Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Received: February 6, 2020 Accepted: April 20, 2020 Published: May 23, 2020
https://doi.org/10.18632/aging.103265How to Cite
Copyright © 2020 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Deficits in coordinated motor behavior and mitochondrial complex V activity have been observed in aged males. Testosterone supplementation can improve coordinated motor behavior in aged males. We investigated the effects of testosterone supplementation on mitochondrial complex V function in the substantia nigra (a brain region that regulates motor activity) in aged male rats. These rats exhibited diminished ATP levels, attenuated mitochondrial complex V activity, and reduced expression of 3 of the 17 mitochondrial complex V subunits (ATP6, ATP8 and ATP5C1) in the substantia nigra. Testosterone supplementation increased ATP levels, mitochondrial complex V activity, and ATP6, ATP8 and ATP5C1 expression in the substantia nigra of the rats. Conversely, orchiectomy reduced mitochondrial complex V activity, downregulated ATP6 and ATP8 expression, and upregulated ATP5C1, ATP5I and ATP5L expression in the substantia nigra. Testosterone replacement reversed those effects. Thus, testosterone enhanced mitochondrial complex V function in the substantia nigra of aged male rats by upregulating ATP6 and ATP8. As potential testosterone targets, these two subunits may to some degree maintain nigrostriatal dopaminergic function in aged males.