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Research Paper|Volume 12, Issue 2|pp 1778—1791

LncRNA DANCR attenuates brain microvascular endothelial cell damage induced by oxygen-glucose deprivation through regulating of miR-33a-5p/XBP1s

Mengqi Zhang1, Mimi Tang2, Qian Wu3, Zhuolu Wang4, Zhuohui Chen1, Hui Ding1, Xinhang Hu1, Xinyi Lv1, Songfeng Zhao1, Jingyan Sun1, Shuntong Kang1, Tong Wu1, Bo Xiao1
  • 1Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China
  • 2Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China
  • 3Department of Neurology, First Affiliated Hospital, Kunming Medical University, Kunming 650032, China
  • 4Department of Breast Surgery, Hunan Provincial Maternal and Child Health Care Hospital, Changsha 410008, China
Received: August 17, 2019Accepted: January 2, 2020Published: January 26, 2020
https://doi.org/10.18632/aging.102712

Copyright: © 2020 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Brain microvascular endothelial cell (BMEC) survival and angiogenesis after ischemic stroke has great significance for improving the prognosis of stroke. Abnormal variants of lncRNAs are closely associated with stroke. In this study, we examined the effects and molecular mechanisms of differentiation antagonizing non-protein coding RNA (DANCR) on apoptosis, migration, and angiogenesis of oxygen-glucose deprivation (OGD)-treated BMECs. We found that DANCR expression significantly increased at 2, 4, 6, 8, and 10 h after OGD. DANCR overexpression promoted cell viability, migration, and angiogenesis in OGD-treated BMECs. Additionally, we found that X-box binding protein l splicing (XBP1s) expression was positively correlated with DANCR expression. DANCR overexpression promoted XBP1s expression in OGD-treated BMECs. Silenced XBP1s reversed the effect of DANCR in OGD-treated BMECs. Furthermore, we found that microRNA (miR)-33a-5p bound to DANCR and the 3'-UTR of XBP1. miR-33a-5p overexpression inhibited proliferation, migration, angiogenesis, and XBP1s expression in OGD-treated DANCR-overexpressing BMECs, reversing the protective effect of DANCR. Finally, we found that XBP1s expression promoted proliferation, migration, and angiogenesis, reversing the damaging effect of miR-33a-5p. In conclusion, DANCR enhanced survival and angiogenesis in OGD-treated BMECs through the miR-33a-5p/XBP1s axis.