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Research Paper|Volume 12, Issue 1|pp 224—241

ASPM promotes glioblastoma growth by regulating G1 restriction point progression and Wnt-β-catenin signaling

Xin Chen1, Lijie Huang2, Yang Yang3, Suhua Chen1, Jianjun Sun1, Changcheng Ma1, Jingcheng Xie1, Yongmei Song4, Jun Yang1
  • 1Department of Neurosurgery, Peking University Third Hospital, Beijing 100191, China
  • 2Beijing Neurosurgical Institute, Capital Medical University, Beijing 100070, China
  • 3Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
  • 4State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
Received: August 11, 2019Accepted: December 5, 2019Published: January 6, 2020
https://doi.org/10.18632/aging.102612

Copyright: © 2020 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Increasing evidence has indicated that the disorganized expression of certain genes promotes tumour progression. In this study, we elucidate the potential key differentially expressed genes (DEGs) between glioblastoma (GBM) and normal brain tissue by analysing three different mRNA expression profiles downloaded from the Gene Expression Omnibus (GEO) database. DEGs were sorted, and key candidate genes and signalling pathway enrichments were analysed. In our analysis, the highest fold change DEG was found to be abnormal spindle-like microcephaly associated (ASPM). The ASPM expression pattern from the database showed that it is highly expressed in GBM tissue, and patients with high expression of ASPM have a poor prognosis. Moreover, ASPM showed aberrantly high expression in GBM cell lines. Loss-of-function assay indicated that ASPM enhances tumorigenesis in GBM cells in vitro. Xenograft growth verified the oncogenic activity of ASPM in vivo. Furthermore, downregulation of ASPM could arrest the cell cycle of GBM cells at the G0/G1 phase and attenuate the Wnt/β-catenin signalling activity in GBM. These data suggest that ASPM may serve as a new target for the therapeutic treatment of GBM.