Research Paper|Volume 11, Issue 23|pp 11504—11519

Resveratrol delays postovulatory aging of mouse oocytes through activating mitophagy

Jilong Zhou^1,², Zhouyiyuan Xue^1,², Hai-Nan He^1,², Xin Liu^1,², Shu-Yuan Yin^1,², Dan-Ya Wu^1,², Xia Zhang^1,³, Heide Schatten⁵, Yi-Liang Miao^1,^2,⁴

¹Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
²Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China
³Experimental Veterinary Medicine Education, Huazhong Agricultural University, Wuhan 430070, China
⁴The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China
⁵Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA

* Equal contribution

Received: September 17, 2019Accepted: November 19, 2019Published: December 13, 2019

https://doi.org/10.18632/aging.102551

Copyright © 2019 Zhou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Resveratrol (3,5,4′-trihydroxystilbene, RSV) is a natural potential anti-aging polyphenolic compound frequently used as a nutritional supplement against several diseases. However, the underlying mechanisms by which resveratrol regulates postovulatory aging of oocytes are still insufficiently known. In this study, we found that resveratrol could delay postovulatory aging and improve developmental competence of oocytes through activating selective mitophagy in the mouse. Resveratrol could maintain spindle morphology but it disturbed cortical granule (CG) distribution during oocyte aging. This might be due to upregulated mitophagy, since blocking mitophagy by cyclosporin A (CsA) treatment affected oocyte quality by damaging mitochondrial function and it decreased embryonic development. In addition, we also observed an involvement of FoxO3a in regulating mitophagy in aging oocytes following resveratrol treatment. Taken together, our results provide evidence that mitophagy induced by resveratrol is a potential mechanism to protect against postovulatory oocyte aging.