Research Paper Volume 11, Issue 23 pp 11157—11169
A twenty gene-based gene set variation score reflects the pathological progression from cirrhosis to hepatocellular carcinoma
- 1 Department of Medical Oncology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China
- 2 Department of Hepatobiliary Surgery, Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China
- 3 The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China
- 4 Department of Endoscopy, Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China
Received: August 29, 2019 Accepted: November 18, 2019 Published: December 6, 2019
https://doi.org/10.18632/aging.102518How to Cite
Copyright © 2019 Lin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The molecular mechanism of the pathological progression from cirrhosis to hepatocellular carcinoma (HCC) remains elusive. In the present study, tissue samples from normal liver, cirrhosis and HCC were subjected to differentially gene expression analysis, weighted gene correlation network analysis to identify the twenty hub genes (TOP2A, CDC20, PTTG1, CDCA5, CCNB2, PRC1, KIF20A, SF3B4, HSP90AB1, FOXD2, PLOD3, CCT3, SETDB1, VPS45, SPDL1, RACGAP1, MED24, KIAA0101, ZNF282, and USP21) in the pathological progression from cirrhosis to HCC. Each sample was calculated a hub gene set variation analysis (HGSVA) score using Gene Set Variation Analysis, The HGSVA score significantly increased with progression from cirrhosis to HCC, and this result was validated in two independent data sets. Moreover, this score may be used as a blood-based marker for HCC and is an independent prognostic factor of recurrence-free survival (RFS) and overall survival (OS). High expression of the hub genes may be driven by hypomethylation. The twenty gene-based gene set variation score may reflect the pathological progression from cirrhosis to HCC and is an independent prognostic factor for both OS and RFS.