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Research Paper|Volume 11, Issue 22|pp 10374—10384

Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Qiang Zhang1, Jingjing Zhang2, Zhanzhao Fu1, Lixin Dong1, Yong Tang3, Chunlei Xu3, Haifeng Wang4, Tao Zhang5, Yue Wu6, Chao Dong7, Shasha Shao1, Guangxia Wang1
  • 1Department of Oncology, First Hospital of Qinhuangdao, Qinhuangdao, China
  • 2Department of CT Scan Room, First Hospital of Qinhuangdao, Qinhuangdao, China
  • 3Department of Gastroenterology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China
  • 4Department of Thoracic and Abdominal Radiotherapy, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China
  • 5Department of Oncology, First Hospital of Lanzhou University, Lanzhou, China
  • 6Department of Surgery, Traditional Chinese Hospital of Lu'an, Lu'an, Anhui, China
  • 7Department of Oncology, First Hospital of Shijiazhuang, Shijiazhuang, China
Received: July 13, 2019Accepted: November 8, 2019Published: November 26, 2019
https://doi.org/10.18632/aging.102462

Copyright © 2019 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Evidence has shown that hypoxia promotes esophageal squamous cell carcinoma (ESCC) growth and metastasis, but the molecular mechanisms underlying that response remain poorly understood. MicroRNAs (miRNAs) are post-transcriptional regulators that participate in various cancer-related processes. Here, we demonstrated that hypoxia along with hypoxia-inducible factor 1α significantly increased expression of miR-10b-3p. Inhibition of miR-10b-3p weakened the effects of hypoxia on ESCC cell proliferation, migration and invasion, while miR-10b-3p overexpression had the opposite effects. Mechanistically, miR-10b-3p acted as cancer-promoting gene by targeting testis specific 10. Using a xenograft model, we observed that administration of miR-10b-3p agomir to tumors enhanced their growth and metastasis in vivo. These findings verified the potent regulatory role played by hypoxia-induced miR-10b-3p expression in ESCC progression. These results suggest that miR-10b-3p may be a useful therapeutic target for treating ESCC.