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Research Paper|Volume 11, Issue 20|pp 8810—8824

Increased calcium channel in the lamina propria of aging rat

Ji Min Kim1, Hyoung-Sam Heo2, Sung-Chan Shin3, Hyun-Keun Kwon3, Jin-Choon Lee4, Eui-Suk Sung4, Hyung-Sik Kim5,6, Gi Cheol Park7, Byung-Joo Lee1,3
  • 1Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea
  • 2Division of Bio-Medical Informatics, Center for Genome Science, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, Republic of Korea
  • 3Department of Otorhinolaryngology-Head and Neck Surgery, Pusan National University School of Medicine, Pusan National University, Busan, Republic of Korea
  • 4, Department of Otorhinolaryngology-Head and Neck Surgery, Pusan National University School of Medicine and Biomedical Research Institute, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea
  • 5Department of Life Science in Dentistry, school of Dentistry, Pusan National University, Yangsan, Republic of Korea
  • 6Institute for Translational Dental Science, Pusan National University, Yangsan, Republic of Korea
  • 7Department of Otolaryngology-Head and Neck Surgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Republic of Korea
* Equal contribution
Received: August 8, 2019Accepted: September 5, 2019Published: October 31, 2019
https://doi.org/10.18632/aging.102284

Copyright © 2019 Kim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The alterations of the extracellular matrix (ECM) in lamina propria of the vocal folds are important changes that are associated with decreased vibrations and increased stiffness in aging vocal fold. The aim of this study was to investigate the differences in gene expression of lamina propria using next generation sequencing (NGS) in young and aging rats and to identify genes that affect aging-related ECM changes for developing novel therapeutic target molecule. Among the 40 genes suggested in the NGS analysis, voltage-gated calcium channels (VGCC) subunit alpha1 S (CACNA1S), VGCC auxiliary subunit beta 1 (CACNB1), and VGCC auxiliary subunit gamma 1 (CACNG1) were increased in the lamina propria of the old rats compared to the young rats. The synthesis of collagen I and III in hVFFs decreased after si-CACNA1S and verapamil treatment. The expression and activity of matrix metalloproteinases (MMP)-1 and -8 were increased in hVFFs after the treatment of verapamil. However, there was no change in the expression of MMP-2 and -9. These results suggest that some calcium channels may be related with the alteration of aging-related ECM in vocal folds. Calcium channel has promising potential as a novel therapeutic target for the remodeling ECM of aging lamina propria.