Research Paper|Volume 11, Issue 2|pp 590—614

Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism

Xing-hua Pan^1,^2,³, Yu-hao Chen^1,^2,³, Yu-kun Yang^1,^2,³, Xue-juan Zhang^1,^2,³, Qing-keng Lin^1,^2,³, Zi-an Li^1,^2,³, Xue-min Cai^1,^2,³, Rong-qing Pang^1,^2,³, Xiang-qing Zhu^1,^2,³, Guang-ping Ruan^1,^2,³

¹Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan Province, China
²Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan Province, China
³Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan Province, China

Received: September 29, 2018Accepted: January 5, 2019Published: January 23, 2019

https://doi.org/10.18632/aging.101762

Copyright: © 2019 Pan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The relationship between bone marrow mesenchymal stem cells (BMSCs) and aging, as well as the antiaging effects of BMSCs, was observed. An aging macaque BMSC model was established. We isolated BMSCs from young and aged macaques and used RT-PCR and Western blot to confirm the aging-related mRNAs and their expression, revealing that TERT, SIRT1 and SIRT6 expression was decreased in the aged BMSCs. The morphology, immunophenotype, differentiation potential, proliferation potential, and antiaging effects of aged and young BMSCs on 293T cells were compared. The expression of aging-related genes and the difference between the secreted cytokines in natural aging and induced aging BMSCs were observed. The transcriptome of peripheral blood mononuclear cells from macaques was analyzed by high-throughput sequencing. Finally, the transcriptional characteristics and regulatory mechanisms of gene transcription in aging macaques were investigated.