Research Paper Volume 10, Issue 1 pp 56—71
NKILA inhibits NF-κB signaling and suppresses tumor metastasis
- 1 Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China
- 2 Department of General Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China
- 3 Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China
Received: November 12, 2017 Accepted: December 17, 2017 Published: January 17, 2018
https://doi.org/10.18632/aging.101359How to Cite
Abstract
The long non-coding RNA (lncRNA) NKILA (nuclear transcription factor NF-κB interacting lncRNA) functions as a suppressor in human breast cancer and tongue cancer. However, the clinical significance and biological roles of NKILA in esophageal squamous cell carcinoma (ESCC) remain unknown. In this study, we showed that NKILA was downregulated in ESCC tissues and cancer cells compared with their normal counterparts. Low NKILA expression correlated with large tumor size and advanced TNM stage, and predicted poor overall and disease-free survival of ESCC patients. Further loss- and gain-of-function assays indicated that NKILA inhibited proliferation and migration of ESCC cells in vitro, suppressed tumor growth and lung metastasis in vivo. Mechanistically, NKILA could inhibit phosphorylation of IκBα, suppress p65 nuclear translocation and downregulate the expression of NF-κB target genes in ESCC cells. These results suggest NKILA could suppress malignant development of ESCC via abrogation of the NF-κB signaling and may potentially serve as a prognostic marker for ESCC.
Abbreviations
ESCC: esophageal squamous cell carcinoma; NKILA: nuclear transcription factor NF-κB interacting lncRNA; NF-κB: Nuclear factor-κB; IκBα: inhibitor of κBα; IKK: IκBα kinase; H&E: hematoxylin and eosin.