Figure 5.Aging-related tissue fibrosis in the gastrocnemius muscles of female mice. Picro-Sirius Red-stained cross-sections of the gastrocnemius muscles from 6- and 24-month-old female mice with PAI-1 gene deficiency (PAI-1−/−) and their wild-type counterparts (PAI-1+/+) are shown (A). Photomicrographs of four regions in each muscle section were analyzed, and the mean occupancy of collagen-containing fibrotic areas in tissue sections was calculated (B) (n = 3 in each group). Scale bars = 100 μm. Data are presented as mean ± standard error of the mean. *P < 0.05.
