Figure 2.Measurements of polyamine levels in wild-type w1118 whole flies in different diets. (A–C) HPLC-MS measurements of spermidine (A–C), spermine (D–F), ornithine (G–I) and putrescine (J–L) in wild-type w1118 whole flies fed with 2% yeast, 12% yeast, 2% yeast + Spd, 12% yeast + Spd at 5-, 15- and 30-day-old time points respectively. n = 9 Biological samples. Each biological sample contains whole flies. Each biological sample has at least 10 whole flies for HPLC-MS measurements. *p < 0.05, **p < 0.01, ***p < 0.001, ****p< 0.0001, ns, not significant. Data are mean ± SEM. P-values were determined 2-way ANOVA with Tukey’s post hoc multiple comparisons test (A–L).
