Research Paper Volume 17, Issue 1 pp 161—169

Figure 2. (A) The figure illustrates the basic components of HD-RAd-STEMCCA-GFP-Tet-Off genome. Abbreviations: GFP: humanized Green Fluorescent Protein; TRE: Tetracycline responsive element; tTA: chimeric regulatory protein; PminCMV: cytomegalovirus minimal promoter; SV40pA: polyadenylation signal; ITR: inverted terminal repeats; ψ: packaging signal. (B) Expression of GFP from the adenovector HD-RAd-STEMCCA-GFP in the third ventricle of the rat hypothalamus 6 days after vector injection in the lateral ventricles. Notice the processes of the ependymal tanycytes forming an extensive network in the hypothalamic parenchyma. Scale bar, 50 µm (for both, B, C). (C) Expression of DsRed2 from the control adenovector HD-RAd-DsRed2 in the rat hypothalamus 30 days after vector injection. Notice the processes of the ependymal tanycytes forming an extensive network in the hypothalamic parenchyma.