Research Paper Volume 16, Issue 21 pp 13181—13200

Figure 1. Proliferation of male and female BMECs increases with female MK CM treatment. (A) Male BMECs treated with male MK CM. (B) Female BMECs treated with female MK CM. (C) Female BMECs treated with male MK CM. (D) Male BMECs treated with female MK CM. The number of cells was quantified following a 48-hour incubation period. Data are expressed as a mean ± SD fold change relative to their respective controls (n = 3–11 biological replicates/group). For the negative control group (α-MEM lacking serum), n = 3 for male and 4 for female BMECs). Significance was determined using a one-way ANOVA with Tukey’s post-hoc test (A, B, D) or Kruskal-Wallis test (C) depending on the normalcy of the data distribution as determined by a Shapiro-Wilk test. Female BMECs treated with young female MK CM, old female MK CM, and young male MK CM proliferated significantly more than control female BMECs. Male BMECs treated with young or old female MK CM exhibited significantly greater proliferation. No significant differences in proliferation were found in male BMECs treated with male MK CM.