Research Paper
Ginsenoside Rh4 reverses ABCB1-induced doxorubicin resistance via the PI3Kδ/AKT axis in osteosarcoma
Figure 7. Rescue experiment validation of ginsenoside Rh4 reversing drug resistance via the PI3K/AKT axis. (A) Drug sensitivity curves for MG63/DXR cells to doxorubicin, illustrating the effects of PI3KCD inhibition or overexpression on drug inhibition efficiency. (B) Western blot analysis of ABCB1 and PI3K/AKT pathway-related protein levels upon PI3KCD inhibition, employing GAPDH as an internal standard to ensure consistent quantification across samples. (C) Western blot analysis detailing the levels of ABCB1 and PI3K/AKT pathway-related proteins following PI3KCD overexpression, with GAPDH used as a baseline for normalization.